Rational design and construction of multi-copy biomanufacturing islands in mammalian cells.

ABSTRACT: Cell line development is a critical step in the establishment of a biopharmaceutical manufacturing process. Current protocols rely on random transgene integration and amplification. Due to considerable variability in transgene integration profiles, this workflow results in laborious screening campaigns before stable producers can be identified. Alternative approaches for transgene dosage increase and integration are therefore highly desirable. In this study, we present a novel strategy for the rapid design, construction, and genomic integration of engineered multiple-copy gene constructs consisting of up to 10 gene expression cassettes. Key to this strategy is the diversification, at the sequence level, of the individual gene cassettes without altering their protein products. We show a com

SUBMITTER: Altamura R 

PROVIDER: S-EPMC8754653 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature