Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.

ABSTRACT: Candida albicans is both a commensal and an opportunistic fungal pathogen. Invading hyphae of C. albicans secrete candidalysin, a pore-forming peptide toxin. To prevent cell death, epithelial cells must protect themselves from direct damage induced by candidalysin and by the mechanical forces exerted by expanding hyphae. We identify two key Ca²⁺-dependent repair mechanisms employed by epithelial cells to withstand candidalysin-producing hyphae. Using camelid nanobodies, we demonstrate candidalysin secretion directly into the invasion pockets induced by elongating C. albicans hyphae. The toxin induces oscillatory increases in cytosolic [Ca²⁺], which cause hydrolysis of PtdIns(4,5)P₂ and loss of cortical actin. Epithelial cells dispose of damaged membrane reg

SUBMITTER: Westman J 

PROVIDER: S-EPMC8755444 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature