Long Tracts of Guanines Drive Aggregation of RNA G-Quadruplexes in the Presence of Spermine.

ABSTRACT: G-Quadruplexes (GQs) are compact, stable structures in DNA and RNA comprised of two or more tiers of quartets whose G-rich motif of tracts of two or more G's occurs commonly within genomes and transcriptomes. While thermodynamically stable in vitro, these structures remain difficult to study in vivo. One approach to understanding GQ in vivo behavior is to test whether conditions and molecules found in cells facilitate their folding. Polyamines are biogenic polycations that interact with RNA. Among common polyamines, spermine contains the highest charge and is found in eukaryotes, making it a good candidate for association with high-charge density nucleic acid structures like GQs. Using a variety of techniques, including ultraviolet-detected thermal denaturation, circul

SUBMITTER: Williams AM 

PROVIDER: S-EPMC8755445 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature