Ontology highlight
ABSTRACT:
SUBMITTER: Zhang Y
PROVIDER: S-EPMC8755756 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Zhang Yanfei Y Cortez Jeremy D JD Hammer Sarah K SK Carrasco-López César C García Echauri Sergio Á SÁ Wiggins Jessica B JB Wang Wei W Avalos José L JL
Nature communications 20220112 1
Branched-chain amino acid (BCAA) metabolism fulfills numerous physiological roles and can be harnessed to produce valuable chemicals. However, the lack of eukaryotic biosensors specific for BCAA-derived products has limited the ability to develop high-throughput screens for strain engineering and metabolic studies. Here, we harness the transcriptional regulator Leu3p from Saccharomyces cerevisiae to develop a genetically encoded biosensor for BCAA metabolism. In one configuration, we use the bio ...[more]