Gastrodin exerts protective effects in reactive TNC1 astrocytes via regulation of the Notch signaling pathway.

ABSTRACT:

Background

Gastrodin (GAS) has been proven to play a therapeutic role in a variety of neurological diseases by affecting activated astrocytes, however, the underlying mechanisms have not been fully illustrated. This study aimed to investigate if GAS exerts the neuroprotective effect through regulating the Notch signaling pathway involved in reactive astrocytes.

Methods

Astrocyte cell lines (TNC1 cells) were cultured in vitro. The hypoxic-ischemic cell model was prepared using the oxygen-glucose deprivation (OGD) method, GAS's pretreatment concentration was 0.34 mM, intervention for 1 hour. Cell counting kit-8 (CCK-8) assay, Transwell migration assay, immunofluorescent staining (double staining), and Western blotting were used to observe the effects of OGD or GAS inter

SUBMITTER: Zhang XLN 

PROVIDER: S-EPMC8756211 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature