Integrated transcriptomic and metabolomic analysis of rat serum to investigate potential target of puerarin in the treatment post-traumatic stress disorder.

ABSTRACT:

Background

Puerarin is a root extract of Pueraria lobate that can alleviate the behavioral disorders and could be therapy for post-traumatic stress disorder (PTSD). However, the underlying mechanism of puerarin in PTSD is unclear, so, we hypothesized that integration of its metabolomic and transcriptomic profiles in rat serum could help to identify the mechanisms of the protective action of puerarin.

Methods

We used the single prolonged stress procedure to establish a model of PTSD in rats and then subjected them to treatment with or without puerarin. Serum metabolomics and transcriptomics were analyzed by ultra-high-performance liquid chromatography-tandem mass spectrometry and mRNA sequencing. The differential metabolites were evaluated by multivariate analysis, and Bayes

SUBMITTER: Su A 

PROVIDER: S-EPMC8756214 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature