Iron metabolism regulation of epithelial-mesenchymal transition in idiopathic pulmonary fibrosis.

ABSTRACT:

Background

Iron overload has been found in the lungs of patients with idiopathic pulmonary fibrosis (IPF) and is thought to be involved in disease progression; however, the underlying mechanism is complex and not yet fully understood. We sought to assess the in vitro role of iron in the progression of fibrosis in lung epithelial cells, and examine the possible regulation of iron and IPF.

Methods

Erastin was used to establish a cell model of iron accumulation in mouse lung epithelial cell line 12 (MLE-12). A Cell Counting Kit-8 assay and annexin V staining were applied to measure cell viability and apoptosis, quantitative polymerase chain reaction (qPCR) and quantitative immunoblot analysis of the protein was conducted to analyze the expression of E-cadherin, N-cadheri

SUBMITTER: Han Y 

PROVIDER: S-EPMC8756254 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature