A photonic crystal fiber-based fluorescence sensor for simultaneous and sensitive detection of lactic acid enantiomers.

ABSTRACT: A photonic crystal fiber (PCF)-based fluorescence sensor is developed for rapid and sensitive detection of lactic acid (LA) enantiomers in serum samples. The sensor is fabricated by chemical binding dual enzymes on the inner surface of the PCF with numerous pore structures and a large specific surface area, which is suitable to be utilized as an enzymatic reaction carrier. To achieve simultaneous detection of L-LA and D-LA, the PCF with an aldehyde-activated surface is cut into two separate pieces, one of which is coated with L-LDH/GPT enzymes and the other with D-LDH/GPT enzymes. By being connected and carefully aligned to each other by a suitable sleeve tube connector, the responses of both L-LA and D-LA sensors are determined by laser-induced flourescence (LIF) detection. With the aid o

SUBMITTER: Chi Z 

PROVIDER: S-EPMC8756416 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature