Antibacterial Nanoparticles with Natural Photosensitizers Extracted from Spinach Leaves.

ABSTRACT: We prepared antibacterial polystyrene nanoparticles (NPs) with natural photosensitizers from chlorophyll (Chl) extract via a simple nanoprecipitation method using the same solvent for dissolution of the polystyrene matrix and extraction of Chls from spinach leaves. A high photo-oxidation and antibacterial effect was demonstrated on Escherichia coli and was based on the photogeneration of singlet oxygen O₂(¹Δ_g), which was directly monitored by NIR luminescence measurements and indirectly verified using a chemical trap. The photoactivity of NPs was triggered by visible light, with enhanced red absorption by Chls. To reduce the quenching effect of carotenoids (β-carotene, lutein, etc.) in the Chl extract, diluted and/or preirradiated samples, in which t

SUBMITTER: Ludacka P 

PROVIDER: S-EPMC8756605 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature