Human amniotic mesenchymal stem cells combined with PPCNg facilitate injured endometrial regeneration.

ABSTRACT:

Background

Caused by the injury to the endometrial basal layer, intrauterine adhesions (IUA) are characterized by uterine cavity obliteration, leading to impaired fertility. Human amniotic mesenchymal stem cells (hAMSCs) have the potential to promote endometrial regeneration mainly through paracrine ability. PPCNg is a thermoresponsive biomaterial consisted of Poly (polyethylene glycol citrate-co-N-isopropylacrylamide) (PPCN) mixed with gelatin, which has been reported as a scaffold for stem cell transplantation. This study aims to investigate the therapeutic effect of hAMSCs combined with PPCNg transplantation in promoting the regeneration of injured endometrium.

Methods

hAMSCs were cultured in different concentrates of PPCNg in vitro, and their proliferation, apoptosis and

SUBMITTER: Huang J 

PROVIDER: S-EPMC8756707 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature