Agitation-dependent biomechanical forces modulate GPVI receptor expression and platelet adhesion capacity during storage.

ABSTRACT:

Background

Continuous agitation during storage slows down the platelet storage lesions. However, in special circumstances, manual-mixing can be alternatively used to store products for short time periods without compromising platelet quality. Based on this finding, and given the role of shear stress in modulating receptor expression, we were interested in comparing the levels of platelet adhesion receptor, GPVI and platelet adhesion capacity under each storage condition.

Methods

Platelet concentrates (PCs) were divided into three groups: continuously-agitated PCs (CAG-PCs) with or without PP2 (Src kinase inhibitor) and manually-mixed PCs (MM-PCs). Platelet count/MPV, swirling, GPVI and P-selectin expression, GPVI shedding, platelet adhesion/spreading to collagen were examine

SUBMITTER: Hosseini E 

PROVIDER: S-EPMC8756730 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature