Biophysical and Structural Characterization of Ribulose-5-phosphate Epimerase from Leishmania donovani.

ABSTRACT: Pentose phosphate pathway (PPP) plays a crucial role in the maintenance of NADPH/NADP⁺ homeostasis and provides protection against oxidative stress through detoxification of the reactive oxygen species. Ribulose-5-phosphate epimerase (RPE) participates in catalysis of the interconversion of ribulose-5-phosphate (Ru5P) to xylulose-5-phosphate (Xu5P) during PPP, however the structural attributes of this enzyme are still underexplored in many human pathogens including leishmanial parasites. The present study focuses upon cloning, purification and characterization of RPE of Leishmania donovani (LdRPE) using various biophysical and structural approaches. Sequence analysis has shown the presence of trypanosomatid-specific insertions at the N-terminus that are absent in h

SUBMITTER: Narsimulu B 

PROVIDER: S-EPMC8756792 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature