Metabolomics activity screening of T cell-induced colitis reveals anti-inflammatory metabolites.

ABSTRACT: Untargeted metabolomics of disease-associated intestinal microbiota can detect quantitative changes in metabolite profiles and complement other methodologies to reveal the full effect of intestinal dysbiosis. Here, we used the T cell transfer mouse model of colitis to identify small-molecule metabolites with altered abundance due to intestinal inflammation. We applied untargeted metabolomics to detect metabolite signatures in cecal, colonic, and fecal samples from healthy and colitic mice and to uncover differences that would aid in the identification of colitis-associated metabolic processes. We provided an unbiased spatial survey of the GI tract for small molecules, and we identified the likely source of metabolites and biotransformations. Several prioritized metabolites that we detected

SUBMITTER: Montenegro-Burke JR 

PROVIDER: S-EPMC8757460 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature