Spatial immune heterogeneity of hypoxia-induced exhausted features in high-grade glioma.

ABSTRACT: The tumor immune microenvironment (TIME) in high-grade glioma (HGG) exhibits high spatial heterogeneity. Though the tumor core and peripheral regions have different biological features, the cause of this spatial heterogeneity has not been clearly elucidated. Here, we examined the spatial heterogeneity of HGG using core and peripheral regions obtained separately from the patients with HGG. We analyzed infiltrating immune cells by flow cytometry from 34 patients with HGG and the transcriptomes by RNA-seq analysis from 18 patients with HGG. Peripheral region-infiltrating immune cells were in vitro cultured in hypoxic conditions and their immunophenotypes analyzed. We analyzed whether the frequencies of exhausted CD8⁺ T cells and immunosuppressive cells in the core or periphe

SUBMITTER: Kim AR 

PROVIDER: S-EPMC8757477 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature