Supplementation of fetal bovine serum increased the quality of in vitro fertilized porcine embryo.

ABSTRACT:

Objective

The present study aimed to explain the effect of fetal bovine serum (FBS) on the in vitro production of porcine embryos and the molecular effects of FBS on the growing of porcine embryos.

Materials and methods

Immature porcine oocytes were matured and fertilized in vitro. The resulting zygotes were cultured in porcine zygotic medium-3- until day 7 and FBS was added on day 4. Without FBS, it was treated as a control group. Quantitative real-time PCR and 2',7'-dichloro-dihydro-fluorescein diacetate (H₂DCFDA) molecular staining techniques were used to detect the expression patterns of apoptosis-associated genes and the accumulation of reactive oxygen species (ROS), respectively. Paired student's t-test was used by GraphPad Prism statist

SUBMITTER: Biswas D 

PROVIDER: S-EPMC8757674 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature