Effects of adipose-derived stromal cells and endothelial progenitor cells on adipose transplant survival and angiogenesis.

ABSTRACT:

Background

A paracrine mechanism is thought to mediate the proangiogenic capacity of adipose-derived stromal/stem cells (ASCs). However, the precise mechanism by which ASCs promote the formation of blood vessels by endothelial progenitor cells (EPCs) is unclear.

Methods

The EPCs-ASCs cocultures prepared in different ratios were subjected to tube formations assay to verify whether ASCs could directly participate in the tube genesis. The supernatant from cultured ASCs was used to stimulate EPCs to evaluate the effects on the angiogenic property of EPCs, as well as capacity for migration and invasion. A coculture model with transwell chamber were used to explore the regulation of angiogenesis markers expression in EPCs by ASCs. We then mixed ASCs with EPCs and transplanted them

SUBMITTER: Gan F 

PROVIDER: S-EPMC8758088 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature