Dedifferentiation alters chondrocyte nuclear mechanics during in vitro culture and expansion.

ABSTRACT: The biophysical features of a cell can provide global insights into diverse molecular changes, especially in processes like the dedifferentiation of chondrocytes. Key biophysical markers of chondrocyte dedifferentiation include flattened cellular morphology and increased stress-fiber formation. During cartilage regeneration procedures, dedifferentiation of chondrocytes during in vitro expansion presents a critical limitation to the successful repair of cartilage tissue. Our study investigates how biophysical changes of chondrocytes during dedifferentiation influence the nuclear mechanics and gene expression of structural proteins located at the nuclear envelope. Through an experimental model of cell stretching and a detailed spatial intranuclear strain quantification, we identified that st

SUBMITTER: Ghosh S 

PROVIDER: S-EPMC8758405 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature