4polar-STORM polarized super-resolution imaging of actin filament organization in cells.

ABSTRACT: Single-molecule localization microscopy provides insights into the nanometer-scale spatial organization of proteins in cells, however it does not provide information on their conformation and orientation, which are key functional signatures. Detecting single molecules' orientation in addition to their localization in cells is still a challenging task, in particular in dense cell samples. Here, we present a polarization-splitting scheme which combines Stochastic Optical Reconstruction Microscopy (STORM) with single molecule 2D orientation and wobbling measurements, without requiring a strong deformation of the imaged point spread function. This method called 4polar-STORM allows, thanks to a control of its detection numerical aperture, to determine both single molecules' localization and ori

SUBMITTER: Rimoli CV 

PROVIDER: S-EPMC8758668 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature