TcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens.

ABSTRACT: Protease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10^-7 mol L^-1. The thermostability analysis of rTcTI showed that 100% inhibitory activity was retained up to 60 °

SUBMITTER: do Amaral M 

PROVIDER: S-EPMC8758671 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature