Bivalent single domain antibody constructs for effective neutralization of Venezuelan equine encephalitis.

ABSTRACT: Venezuelan equine encephalitis virus (VEEV) is a mosquito borne alphavirus which leads to high viremia in equines followed by lethal encephalitis and lateral spread to humans. In addition to naturally occurring outbreaks, VEEV is a potential biothreat agent with no approved human vaccine or therapeutic currently available. Single domain antibodies (sdAb), also known as nanobodies, have the potential to be effective therapeutic agents. Using an immune phage display library derived from a llama immunized with an equine vaccine that included inactivated VEEV, five sdAb sequence families were identified that showed varying ability to neutralize VEEV. One of the sequence families had been identified previously in selections against chikungunya virus, a related alphavirus of public health concer

SUBMITTER: Liu JL 

PROVIDER: S-EPMC8758676 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature