Dataset Information

Endophytism of Fusarium Solani in Meristems of Delonix Regia (Bojer ex Hook.) Raf.

ABSTRACT: A bright yellow pigment-producing endophytic fungus strain GMRS1 was isolated from the meristems of Delonix regia (Bojer ex Hook.) Raf. The fungus was identified as Fusarium solani on grounds of morphological and molecular analysis. The chemical composition of the crude pigments was partially analyzed by Quadrupole Time-of-flight Liquid chromatography/Mass spectrometry/Mass spectrometry and the major compounds were reported. The crude pigments were proved toxic and unsafe by acute oral toxicity study on Wistar rats. The total cholesterol and glucose levels of the rats ingested with crude pigments were significantly elevated than the control rats. The treatment rats were further observed with damaged liver and kidney tissues. The LD₅₀ value of the crude pigments was higher than 1000 mg/kg of body weight.

Supplementary information

The online version contains supplementary material available at 10.1007/s12088-021-00968-w.

SUBMITTER: Vijayakumar S

PROVIDER: S-EPMC8758843 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Endophytism of Fusarium Solani in Meristems of Delonix Regia (Bojer ex Hook.) Raf.

Vijayakumar Sujithra S Ramamoorthy Siva S

Indian journal of microbiology 20210809 1

A bright yellow pigment-producing endophytic fungus strain GMRS1 was isolated from the meristems of Delonix regia (Bojer ex Hook.) Raf. The fungus was identified as Fusarium solani on grounds of morphological and molecular analysis. The chemical composition of the crude pigments was partially analyzed by Quadrupole Time-of-flight Liquid chromatography/Mass spectrometry/Mass spectrometry and the major compounds were reported. The crude pigments were proved toxic and unsafe by acute ...[more]

PMID: 35068613

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Project description:Purpose: Delonix regia or Gulmohor commonly grows here and there in Indian villages as well as it is used for megacity beautification and environmental management due to its evergreen nature and vibrant flower colour. However, an increasing incidence of seasonal pollinosis was observed among the inhabitants living in close vicinity to this tree suggesting a possible link between the airborne pollen load and the concomitant respiratory hazards. This prompted us to investigate the allergens in the pollen of this dominant avenue tree. Methodology: Allergenicity of D. regia pollen grains was first checked by Skin Prick Test (SPT) and further confirmed by in vitro tests, such as, ELISA, Dot Blot and histamine release assay. The total proteome profiling was done by 2D PAGE and it was confronted with the pooled sera of 10 patients. The IgE reactive proteins were identified by MALDI TOF/TOF in Autoflex speed (Bruker,Germany). The raw spectra were searched against NCBInr database using MASCOT search engine. Result: Delonix regia, pollen grains have been found in considerable amount in the air during its flowering season (May to July). Approximately 31% of atopic individuals were found allergic to D. regia, pollen with elevated level of specific IgE and histamine in the serum. Total 8 IgE reactive proteins have been identified by homology driven proteomics. These proteins are ATP synthase beta subunit (spot no 5), Actin (spot no 6), Hypothetical actin like protein (spot no 7), Recombinant S-adenosylmethionine synthase 2 (spot no 9), UDP-arabinopyranose mutase (spot no 13), Luminal-binding 5 (spot no 2), ELF3-like protein (spot no 8) and hypothetical protein OsJ_04810 (spot no 11). Among these eight identified allergenic proteins, five were previously reported as allergen from different sources whereas the rest three have been reported for the first time as novel allergens. Conclusion: Novelty of this study was to identify 8 allergens from D.regia for the first time using immune-biochemical and proteomic techniques. Further studies will open up new avenues in component resolved diagnosis of pollen allergy.

Dataset Information

Endophytism of Fusarium Solani in Meristems of Delonix Regia (Bojer ex Hook.) Raf.

Supplementary information

Publications

Endophytism of <i>Fusarium Solani</i> in Meristems of <i>Delonix Regia</i> (Bojer ex Hook.) Raf.

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