Chemically modified magnetic immobilized phospholipase A₁ and its application for soybean oil degumming.

ABSTRACT: In this paper, the free Phospholipase A₁ (PLA₁) was immobilized on a magnetic carrier. The average particle diameter of the magnetic carrier was 97 ± 1.3 nm, and the average particle diameter of the magnetically immobilized PLA₁ was 105 nm ± 1.3 nm. The enzyme activity was 1940.5 U/g. The magnetic enzyme was chemically modified with formaldehyde, dextran-aldehyde, and dextran-aldehyde-glycine. The proportions of primary amino groups in the modified magnetic immobilized enzyme PLA₁ were 0, 53.5% and 47.3%, respectively. The optimum pH of the enzyme after chemical modification was 6.5. When the system temperature was 60 °C, the magnetically immobilized PLA₁ modified with dextran-aldehyde-glycine had the optimal activity and stability. Th

SUBMITTER: Weining W 

PROVIDER: S-EPMC8758861 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature