Degradome comparison between wild and cultivated rice identifies differential targeting by miRNAs.

ABSTRACT:

Background

Small non-coding (s)RNAs are involved in the negative regulation of gene expression, playing critical roles in genome integrity, development and metabolic pathways. Targeting of RNAs by ribonucleoprotein complexes of sRNAs bound to Argonaute (AGO) proteins results in cleaved RNAs having precise and predictable 5` ends. While tools to study sliced bits of RNAs to confirm the efficiency of sRNA-mediated regulation are available, they are sub-optimal. In this study, we provide an improvised version of a tool with better efficiency to accurately validate sRNA targets.

Results

Here, we improvised the CleaveLand tool to identify additional micro (mi)RNA targets that belong to the same family and also other targets within a specified free energy cut-off. These additional

SUBMITTER: Swetha C 

PROVIDER: S-EPMC8759253 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature