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ABSTRACT:
SUBMITTER: Altaib H
PROVIDER: S-EPMC8759359 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Altaib Hend H Ozaki Yuka Y Kozakai Tomoya T Sakaguchi Kouta K Nomura Izumi I Suzuki Tohru T
Microbiology resource announcements 20220113 1
A series of <i>Bifidobacterium</i>-Escherichia coli shuttle vectors (pKO403-<i>lacZ'</i>-Cm, pKO403-<i>lacZ'</i>-Sp, pKO403-<i>lacZ'</i>-p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the <i>lacZ'</i>α fragment, overhung by two facing type IIS restriction sites, for blue-white selection and seamless gene cloning. These vectors are useful for gene knockout or multigene integration into the chromosome of <i>Bifidob ...[more]