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Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium.


ABSTRACT: A series of Bifidobacterium-Escherichia coli shuttle vectors (pKO403-lacZ'-Cm, pKO403-lacZ'-Sp, pKO403-lacZ'-p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the lacZ'α fragment, overhung by two facing type IIS restriction sites, for blue-white selection and seamless gene cloning. These vectors are useful for gene knockout or multigene integration into the chromosome of Bifidobacterium.

SUBMITTER: Altaib H 

PROVIDER: S-EPMC8759359 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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<i>Bifidobacterium</i>-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in <i>Bifidobacterium</i>.

Altaib Hend H   Ozaki Yuka Y   Kozakai Tomoya T   Sakaguchi Kouta K   Nomura Izumi I   Suzuki Tohru T  

Microbiology resource announcements 20220113 1


A series of <i>Bifidobacterium</i>-Escherichia coli shuttle vectors (pKO403-<i>lacZ'</i>-Cm, pKO403-<i>lacZ'</i>-Sp, pKO403-<i>lacZ'</i>-p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the <i>lacZ'</i>α fragment, overhung by two facing type IIS restriction sites, for blue-white selection and seamless gene cloning. These vectors are useful for gene knockout or multigene integration into the chromosome of <i>Bifidob  ...[more]

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