In vivo imaging of the human eye using a 2-photon-excited fluorescence scanning laser ophthalmoscope.

ABSTRACT: BackgroundNoninvasive assessment of metabolic processes that sustain regeneration of human retinal visual pigments (visual cycle) is essential to improve ophthalmic diagnostics and to accelerate development of new treatments to counter retinal diseases. Fluorescent vitamin A derivatives, which are the chemical intermediates of these processes, are highly sensitive to UV light; thus, safe analyses of these processes in humans are currently beyond the reach of even the most modern ocular imaging modalities.MethodsWe present a compact, 2-photon-excited fluorescence scanning laser ophthalmoscope and spectrally resolved images of the human retina based on 2-photon excitation (TPE) with near-infrared light. A custom Er:fiber laser with integrated pulse selection, along with intelligent postproce

SUBMITTER: Boguslawski J 

PROVIDER: S-EPMC8759795 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature