RNA Sequencing Analysis of Gene Expression by Electroacupuncture in Guinea Pig Gallstone Models.

ABSTRACT:

Background

Clinical studies have shown that electroacupuncture (EA) promotes gallbladder motility and alleviates gallstone. However, the mechanism underlying the effects of EA on gallstone is poorly understood. In this study, the mRNA transcriptome analysis was used to study the possible therapeutic targets of EA.

Methods

Hartley SPF guinea pigs were employed for the gallstone models. Illumina NovaSeq 6000 platform was used for the RNA sequencing of guinea pig gallbladders in the normal group (Normal), gallstone model group (Model), and EA-treated group (EA). Differently expressed genes (DEGs) were examined separately in Model vs. Normal and EA vs. Model. DEGs reversed by EA were selected by comparing the DEGs of Model vs. Normal and EA vs. Model. Biological functions were e

SUBMITTER: Hao M 

PROVIDER: S-EPMC8759925 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature