Optimized Doxycycline-Inducible Gene Expression System for Genetic Programming of Tumor-Targeting Bacteria.

ABSTRACT:

Purpose

In the programming of tumor-targeting bacteria, various therapeutic or reporter genes are expressed by different gene-triggering strategies. Previously, we engineered pJL87 plasmid with an inducible bacterial drug delivery system that simultaneously co-expressed two genes for therapy and imaging by a bidirectional tet promoter system only in response to the administration of exogenous doxycycline (Doxy). In this multi-cassette expression approach, tetA promoter (P_tetA) was 100-fold higher in expression strength than tetR promoter (P_tetR). In the present study, we developed pJH18 plasmid with novel Doxy-inducible gene expression system based on a tet promoter.

Procedures

In this system, Tet repressor (TetR) expressed by a weak constitutive promot

SUBMITTER: Nguyen DH 

PROVIDER: S-EPMC8760206 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature