Evaluation of immunomodulatory and antioxidant properties of traditional Kwath, conventional extracts of plants Cocculus hirsutus and Cuscuta reflexa - in vitro &ex vivo studies.

ABSTRACT:

Introduction

The consumption of 'Patalagarudi' (Cocculus hirsutus 'CHP') and 'Amarbel' (Cuscuta reflexa 'CRA') as ethnic plants for health promotions rarely validated. The limited literature reported these plants as antioxidant and immunomodulators.

Objective

To evaluate the biodynamic properties of CHP and CRA extracts.

Methodology

The traditional formulation, 'Kwath' (K) and conventional extracts were prepared with CRA and CHP. The total phenolic content (TPC) was estimated. Various polyphenol compounds in the extracts were eluted on UHPLC. The biodynamic activities; i. Free radical scavenging (FRS-DPPH and ABTS), ii. Intracellular ROS scavenging activity in RAW 264.7 cell line iii. Spleenocytes proliferation assay for Th1/Th2 Immunomodulatory potential by flow-cyto

SUBMITTER: Singh A 

PROVIDER: S-EPMC8760447 | biostudies-literature | 2022 Jan-Mar

REPOSITORIES: biostudies-literature