A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing.

ABSTRACT:

Background

miRNAs are regulatory transcripts established as repressors of mRNA stability and translation that have been functionally implicated in carcinogenesis. miR-10b is one of the key onco-miRs associated with multiple forms of cancer. Malignant gliomas exhibit particularly striking dependence on miR-10b. However, despite the therapeutic potential of miR-10b targeting, this miRNA's poorly investigated and largely unconventional properties hamper the clinical translation.

Methods

We utilized Covalent Ligation of Endogenous Argonaute-bound RNAs and their high-throughput RNA sequencing to identify miR-10b interactome and a combination of biochemical and imaging approaches for target validation. They included Crosslinking and RNA immunoprecipitation with spliceosomal protei

SUBMITTER: El Fatimy R 

PROVIDER: S-EPMC8760648 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature