Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907.

ABSTRACT: The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium Bacillus smithii IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The whole cells of the bacterium displayed versatile substrate specificity by exhibiting significant activity with a diverse range of amides. In addition, amidase from thermophilic bacterium was induced by adding Ɛ-caprolactam in the mineral base media. The optimum temperature and pH of acyltransferase activity of amidase enzyme were found to be 50 °C and 7.0, respectively. Interestingly, half-life (t _1/2) of this enzyme was 17.37 h at 50 °C. Bench-scale production and purificati

SUBMITTER: Sharma H 

PROVIDER: S-EPMC8761202 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature