Dataset Information

RNA-seq and phytohormone analysis reveals the culm color variation of Bambusa oldhamii Munro.

ABSTRACT:

Background

The clumping bamboo Bambusa oldhamii Munro, known as "green bamboo", is famous for its edible bamboo shoots and fast-growing timber. The green and yellow striped-culm B. oldhamii variety, named B. oldhamii f. revoluta W.T. Lin & J. Y. Lin, is an attractive system for researching the culm color variation of B. oldhamii.

Methods

Millions of clean reads were generated and assembled into 604,900 transcripts, and 383,278 unigenes were acquired with RNA-seq technology. The quantification of ABA, IAA, JA, GA₁, GA₃, GA₄, and GA₇ was performed using HPLC-MS/MS platforms.

Results

Differential expression analysis showed that 449 unigenes were differentially expressed genes (DEGs), among which 190 DEGs were downregulated and 259 DEGs were upregulated in B. oldhamii f. revoluta. Phytohormone contents, especially GA₁ and GA_7, were higher in B. oldhamii. Approximately 21 transcription factors (TFs) were differentially expressed between the two groups: the bZIP, MYB, and NF-YA transcription factor families had the most DEGs, indicating that those TFs play important roles in B. oldhamii culm color variation. RNA-seq data were confirmed by quantitative RT-PCR analysis of the selected genes; moreover, phytohormone contents, especially those of ABA, GA₁ and GA₇, were differentially accumulated between the groups. Our study provides a basal gene expression and phytohormone analysis of B. oldhamii culm color variation, which could provide a solid fundamental theory for investigating bamboo culm color variation.

SUBMITTER: Jiao Y

PROVIDER: S-EPMC8761368 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

RNA-seq and phytohormone analysis reveals the culm color variation of <i>Bambusa oldhamii</i> Munro.

Jiao Yulian Y Zeng Hu H Xia Haitao H Wang Yueying Y Wang Jinwang J Jin Chuan C

PeerJ 20220113

<h4>Background</h4>The clumping bamboo <i>Bambusa oldhamii</i> Munro, known as "green bamboo", is famous for its edible bamboo shoots and fast-growing timber. The green and yellow striped-culm <i>B. oldhamii</i> variety, named <i>B. oldhamii</i> f. <i>revoluta</i> W.T. Lin & J. Y. Lin, is an attractive system for researching the culm color variation of <i>B. oldhamii</i>.<h4>Methods</h4>Millions of clean reads were generated and assembled into 604,900 transcripts, and 383,278 unigenes were acqui ...[more]

PMID: 35070510

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