ITRAQ-based proteomic analysis of differentially expressed proteins in sera of seronegative and seropositive rheumatoid arthritis patients.

ABSTRACT:

Objective

The diagnosis of seronegative rheumatoid arthritis (SNRA) is often difficult due to the unavailability of reliable laboratory markers. The aim of this study was to identify differentially expressed proteins in sera of SNRA, seropositive RA (SPRA), and healthy donors (HD).

Methods

A total of 32 seropositive RA patients, 32 SNRA patients, and 35 HD were enrolled in our study. Differentially expressed proteins between 3 groups were identified via isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis, and an ELISA test was used for the validation test. Correlation analysis was conducted by GraphPad Prism.

Results

Using iTRAQ quantitative proteomics, we identified 14 proteins were significantly different between SPRA and SNRA, incl

SUBMITTER: He Y 

PROVIDER: S-EPMC8761432 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature