Microarray analysis of circRNAs sequencing profile in exosomes derived from bone marrow mesenchymal stem cells in postmenopausal osteoporosis patients.

ABSTRACT:

Introduction

Bone marrow-derived mesenchymal stem cells (BMSCs)-derived exosomes are involved in the modulation of tissue repair and regeneration. CircRNAs play important roles in BMSCs exosomes. The current study sought to explore the role of circRNAs in exosomes derived from BMSCs of postmenopausal osteoporosis (PMOP) patients and the underlying mechanisms.

Methods

RNA was extracted from BMSCs exosomes of PMOP and a control group. RNA microarray and bioinformatics analyses were used to explore the expression profile and functions circRNAs. Differentially expressed circRNAs from 20 PMOP and 20 controls were analyzed using RT-qPCR.

Results

A total of 237 upregulated and 279 downregulated circRNAs were identified in the current study. The top-10 most upregulated circRN

SUBMITTER: Fu M 

PROVIDER: S-EPMC8761433 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature