Spatial Distribution and Predictive Significance of Dendritic Cells and Macrophages in Esophageal Cancer Treated With Combined Chemoradiotherapy and PD-1 Blockade.

ABSTRACT:

Background

The first clinical study (NCT03671265) of first-line chemoradiotherapy combined with PD-1 blockade showed promising treatment outcomes in locally advanced esophageal squamous cell carcinoma (ESCC). However, partial patients did not respond to the combination treatment. The roles of dendritic cells (DCs) and macrophages in this combination treatment remain poorly understood.

Methods

We performed multiplexed immunofluorescence method to identify CD11c⁺ DCs, CD68⁺ macrophages, and their PD-L1^- or PD-L1⁺ subpopulations in paired tumor biopsies (n = 36) collected at baseline and during the combination treatment (after radiation, 40 Gy) from the phase Ib trial (NCT03671265). We applied whole exome sequencing in the base

SUBMITTER: Ma X 

PROVIDER: S-EPMC8761740 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature