Spatial and Temporal Heterogeneity of Tumor-Infiltrating Lymphocytes in Advanced Urothelial Cancer.

ABSTRACT: Checkpoint inhibitors targeting PD-(L)1 induce objective responses in 20% of patients with metastatic urothelial cancer (UC). CD8⁺ T cell infiltration has been proposed as a putative biomarker for response to checkpoint inhibitors. Nevertheless, data on spatial and temporal heterogeneity of tumor-infiltrating lymphocytes in advanced UC are lacking. The major aims of this study were to explore spatial heterogeneity for lymphocyte infiltration and to investigate how the immune landscape changes during the disease course. We performed multiplex immunohistochemistry to assess the density of intratumoral and stromal CD3⁺, CD8⁺, FoxP3⁺ and CD20⁺ immune cells in longitudinally collected samples of 49 UC patients. Within these samples, spatial

SUBMITTER: van Wilpe S 

PROVIDER: S-EPMC8761759 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature