Analysis by Metabolomics and Transcriptomics for the Energy Metabolism Disorder and the Aryl Hydrocarbon Receptor Activation in Male Reproduction of Mice and GC-2spd Cells Exposed to PM_2.5.

ABSTRACT: Fine particulate matter (PM_2.5)-induced male reproductive toxicity arouses global public health concerns. However, the mechanisms of toxicity remain unclear. This study aimed to further investigate toxicity pathways by exposure to PM_2.5in vitro and in vivo through the application of metabolomics and transcriptomics. In vitro, spermatocyte-derived GC-2spd cells were treated with 0, 25, 50, 100 μg/mL PM_2.5 for 48 h. In vivo, the real-world exposure of PM_2.5 for mouse was established. Forty-five male C57BL/6 mice were exposed to filtered air, unfiltered air, and concentrated ambient PM_2.5 in Tangshan of China for 8 weeks, respectively. The results in vitro and in vivo showed that PM_2.5

SUBMITTER: Shi F 

PROVIDER: S-EPMC8761788 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature