A Semi-Quantitative Assay to Measure Glycosaminoglycan Degradation by the Urinary Microbiota.

ABSTRACT: Glycosaminoglycans (GAGs) are linear polysaccharides and are among the primary components of mucosal surfaces in mammalian systems. The GAG layer lining the mucosal surface of the urinary tract is thought to play a critical role in urinary tract homeostasis and provide a barrier against urinary tract infection (UTI). This key component of the host-microbe interface may serve as a scaffolding site or a nutrient source for the urinary microbiota or invading pathogens, but its exact role in UTI pathogenesis is unclear. Although members of the gut microbiota have been shown to degrade GAGs, the utilization and degradation of GAGs by the urinary microbiota or uropathogens had not been investigated. In this study, we developed an in vitro plate-based assay to measure GAG degradation and u

SUBMITTER: Nguyen VH 

PROVIDER: S-EPMC8762050 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature