Nanoenviroments of the β-Subunit of L-Type Voltage-Gated Calcium Channels in Adult Cardiomyocytes.

ABSTRACT: In cardiomyocytes, Ca²⁺ influx through L-type voltage-gated calcium channels (LTCCs) following membrane depolarization regulates crucial Ca²⁺-dependent processes including duration and amplitude of the action potentials and excitation-contraction coupling. LTCCs are heteromultimeric proteins composed of the Ca_vα₁, Ca_vβ, Ca_vα₂δ and Ca_vγ subunits. Here, using ascorbate peroxidase (APEX2)-mediated proximity labeling and quantitative proteomics, we identified 61 proteins in the nanoenvironments of Ca_vβ₂ in cardiomyocytes. These proteins are involved in diverse cellular functions such as cellular trafficking, cardiac contraction, sarcomere organization and excitation-contraction couplin

SUBMITTER: Cruz-Garcia Y 

PROVIDER: S-EPMC8762238 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature