Transplantation of a human induced pluripotent stem cell-derived airway epithelial cell sheet into the middle ear of rats.

ABSTRACT:

Introduction

Early postoperative regeneration of the middle ear mucosa is essential for the prevention of postoperative refractory otitis media and recurrent cholesteatoma. As a means for intractable otitis media management, we focused on human induced pluripotent stem cell (hiPSC)-derived airway epithelial cells (AECs), which have been used in upper airway mucosal regeneration and transplantation therapy. In this study, we transplanted hiPSC-derived AECs into the middle ear of immunodeficient rats.

Methods

Following the preparation of AEC sheets from hiPSCs, the bilateral middle ear mucosa of X-linked severe combined immunodeficient rats was scraped, and the AEC sheets were transplanted in the ears unilaterally.

Results

Human nuclear antigen (HNA)-positive ciliated c

SUBMITTER: Tada T 

PROVIDER: S-EPMC8762358 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature