Malondialdehyde-Modified Photoreceptor Outer Segments Promote Choroidal Neovascularization in Mice.

ABSTRACT:

Purpose

This study aimed to establish a novel choroidal neovascularization (CNV) mouse model through subretinally injecting malondialdehyde (MDA)-modified photoreceptor outer segments (POS), which was more consistent with the pathogenesis of wet age-related macular degeneration (AMD).

Methods

MDA-modified POS were subretinally injected in C57BL/6J mice. Four weeks later, to assess the volume of CNV and the morphology of retinal pigment epithelium (RPE), isolectin B4 and zonula occludens-1 antibody were used for immunostaining. Fundus fluorescent angiography and optical coherence tomography imaging were used to describe the morphologic features of CNV. Transepithelial resistance was measured on polarized ARPE-19 cells. Vascular endothelial growth factor levels in the cell cul

SUBMITTER: Chen Y 

PROVIDER: S-EPMC8762676 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature