Diversity of bacteriocins in the microbiome of the Tucurui Hydroelectric Power Plant water reservoir and three-dimensional structure prediction of a zoocin.

ABSTRACT: Bacteriocins are antimicrobial peptides expressed by bacteria through ribosomal activity. In this study, we analyzed the diversity of bacteriocin-like genes in the Tucuruí-HPP using a whole-metagenome shotgun sequencing approach. Three layers of the water column were analyzed (photic, aphotic and sediment). Detection of bacteriocin-like genes was performed with blastx using the BAGEL4 database as subject sequences. In order to calculate the abundance of bacteriocin-like genes we also determined the number of 16S rRNA genes using blastn. Taxonomic analysis was performed using RAST server and the metagenome was assembled using IDBA-UD in order to recover the full sequence of a zoocin which had its three-dimensional structure determined. The photic zone presented the highest number of reads a

SUBMITTER: Costa SS 

PROVIDER: S-EPMC8762718 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature