Single Quadrupole Multiple Fragment Ion Monitoring Quantitative Mass Spectrometry.

ABSTRACT: Single quadrupole mass spectrometry (MS) with enhanced in-source multiple fragment ion monitoring was designed to perform high sensitivity quantitative mass analyses. Enhanced in-source fragmentation amplifies fragmentation from traditional soft electrospray ionization producing fragment ions that have been found to be identical to those generated in tandem MS. We have combined enhanced in-source fragmentation data with criteria established by the European Union Commission Directive 2002/657/EC for electron ionization single quadrupole quantitative analysis to perform quantitative analyses. These experiments were performed on multiple types of complex samples that included a mixture of 50 standards, as well as cell and plasma extracts. The dynamic range for these quantitative analyses was

SUBMITTER: Xue J 

PROVIDER: S-EPMC8762722 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature