High-resolution imaging and identification of biomolecules using Nano-DESI coupled to ion mobility spectrometry.

ABSTRACT: Simultaneous spatial localization and structural characterization of molecules in complex biological samples currently represents an analytical challenge for mass spectrometry imaging (MSI) techniques. In this study, we describe a novel experimental platform, which substantially expands the capabilities and enhances the depth of chemical information obtained in high spatial resolution MSI experiments performed using nanospray desorption electrospray ionization (nano-DESI). Specifically, we designed and constructed a portable nano-DESI MSI platform and coupled it with a drift tube ion mobility (IM) spectrometer-mass spectrometer. We demonstrate imaging of drift time-separated ions with a high spatial resolution of better than ∼25 μm using uterine tissues on day 4 of pregnancy in mice. Colli

SUBMITTER: Unsihuay D 

PROVIDER: S-EPMC8762739 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature