NBD-based synthetic probes for sensing small molecules and proteins: design, sensing mechanisms and biological applications.

ABSTRACT: Compounds with a nitrobenzoxadiazole (NBD) skeleton exhibit prominent useful properties including environmental sensitivity, high reactivity toward amines and biothiols (including H2S) accompanied by distinct colorimetric and fluorescent changes, fluorescence-quenching ability, and small size, all of which facilitate biomolecular sensing and self-assembly. Amines are important biological nucleophiles, and the unique activity of NBD ethers with amines has allowed for site-specific protein labelling and for the detection of enzyme activities. Both H2S and biothiols are involved in a wide range of physiological processes in mammals, and misregulation of these small molecules is associated with numerous diseases including cancers. In this review, we focus on NBD-based synthetic probes as advan

SUBMITTER: Jiang C 

PROVIDER: S-EPMC8763210 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature