Generation and diversification of recombinant monoclonal antibodies.

ABSTRACT: Antibodies are indispensable tools used for a large number of applications in both foundational and translational bioscience research; however, there are drawbacks to using traditional antibodies generated in animals. These include a lack of standardization leading to problems with reproducibility, high costs of antibodies purchased from commercial sources, and ethical concerns regarding the large number of animals used to generate antibodies. To address these issues, we have developed practical methodologies and tools for generating low-cost, high-yield preparations of recombinant monoclonal antibodies and antibody fragments directed to protein epitopes from primary sequences. We describe these methods here, as well as approaches to diversify monoclonal antibodies, including customization

SUBMITTER: DeLuca KF 

PROVIDER: S-EPMC8763395 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature