Low-invasive 5D visualization of mitotic progression by two-photon excitation spinning-disk confocal microscopy.

ABSTRACT: Non-linear microscopy, such as multi-photon excitation microscopy, offers spatial localities of excitations, thereby achieving 3D cross-sectional imaging with low phototoxicity even in thick biological specimens. We had developed a multi-point scanning two-photon excitation microscopy system using a spinning-disk confocal scanning unit. However, its severe color cross-talk has precluded multi-color simultaneous imaging. Therefore, in this study, we introduced a mechanical switching system to select either of two NIR laser light pulses and an image-splitting detection system for 3- or 4-color imaging. As a proof of concept, we performed multi-color fluorescent imaging of actively dividing human HeLa cells and tobacco BY-2 cells. We found that the proposed microscopy system enabled time-laps

SUBMITTER: Kamada T 

PROVIDER: S-EPMC8764092 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature