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Acid increases PGE₂ in the duodenal mucosa in rats.

ABSTRACT: Attention has recently been paid to the duodenum as the pathophysiologic center of functional dyspepsia. However, the precise mechanisms of symptom generation remain unknown. We here investigated the effect of acid on duodenal prostaglandin E₂ and localization of prostaglandin E₂ related receptors. Sprague-Dawley rats were used for this study. Hydrochloric acid was administered in the duodenum, then prostaglandin E₂ levels in the duodenum were measured using the ELISA. The expression and localization of prostaglandin receptors (EP1-4) and the mRNAs of prostaglandin synthases were investigated using in situ hybridization histochemistry in duodenal tissue. After acid perfusion, prostaglandin E₂ levels in the duodenum significantly increased. EP3 was expressed mainly at the myenteric plexus in the duodenal mucosa, and EP4 at both the epithelial surface and myenteric plexus. Contrary, EP2 was sparsely distributed in the villi and EP1 were not clearly seen on in situ hybridization histochemistry. Prostaglandin-synthetic enzymes were also distributed in the duodenal mucosa. The prostaglandin E₂ levels in the duodenum increased after acidification. Prostaglandin E₂ receptors and prostaglandin E₂-producing enzymes were both observed in rat duodenum. These observations suggest that duodenal prostaglandin E₂ possibly play a role in the symptom generation of functional dyspepsia.

SUBMITTER: Fujimura T

PROVIDER: S-EPMC8764112 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Acid increases PGE2 in the duodenal mucosa in rats.

Fujimura Tadahiro T Kondo Takashi T Kobayashi Kimiko K Duan Shaoqi S Kanda Hirosato H Kono Tomoaki T Fukushima Masashi M Tomita Toshihiko T Oshima Tadayuki T Fukui Hirokazu H Fujii Yoshihito Y Konemura Takashi T Okada Hiroki H Yamanaka Hiroki H Dai Yi Y Noguchi Koichi K Miwa Hiroto H

Journal of clinical biochemistry and nutrition 20210807 1

Attention has recently been paid to the duodenum as the pathophysiologic center of functional dyspepsia. However, the precise mechanisms of symptom generation remain unknown. We here investigated the effect of acid on duodenal prostaglandin E2 and localization of prostaglandin E2 related receptors. Sprague-Dawley rats were used for this study. Hydrochloric acid was administered in the duodenum, then prostaglandin E2 levels in the duodenum were measured using the ...[more]

PMID: 35068678

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Project description:BackgroundCigarette smoking is a known risk factor in a number of gastrointestinal (GI) diseases in which the microbiota is implicated, including duodenal ulcer and Crohn's disease. Smoking has the potential to alter the microbiota; however, to date, the impact of smoking on the mucosa-associated microbiota (MAM), and particularly that of the upper GI tract, remains very poorly characterised. Thus, we investigated the impact of smoking on the upper small intestinal MAM. A total of 102 patients undergoing upper GI endoscopy for the assessment of GI symptoms, iron deficiency, or Crohn's disease, but without identifiable lesions in the duodenum, were recruited. Smoking status was determined during clinical assessment and patients classified as current (n = 21), previous smokers (n = 40), or having never smoked (n = 41). The duodenal (D2) MAM was profiled via 16S rRNA gene amplicon sequencing.ResultsSmoking, both current and previous, is associated with significantly reduced bacterial diversity in the upper small intestinal mucosa, as compared to patients who had never smoked. This was accompanied by higher relative abundance of Firmicutes, specifically Streptococcus and Veillonella spp. The relative abundance of the genus Rothia was also observed to be greater in current smokers; while in contrast, levels of Prevotella and Neisseria were lower. The MAM profiles and diversity of previous smokers were observed to be intermediate between current and never smokers. Smoking did not impact the total density of bacteria present on the mucosa.ConclusionsThese data indicate the duodenal MAM of current smokers is characterised by reduced bacterial diversity, which is partially but not completely restored in previous smokers. While the precise mechanisms remain to be elucidated, these microbiota changes may in some part explain the adverse effects of smoking on mucosa-associated diseases of the GI tract. Smoking status requires consideration when interpreting MAM data.

Dataset Information

Acid increases PGE₂ in the duodenal mucosa in rats.

Publications

Acid increases PGE<sub>2</sub> in the duodenal mucosa in rats.

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