The Mlc1 Promoter Directs Muller Cell-specific Gene Expression in the Retina.

ABSTRACT:

Purpose

Because the importance of glia in regulating brain functions has been demonstrated, genetic technologies that manipulate glial cell-specific gene expression in the brain have become essential and have made great progress. However, it is unknown whether the same strategy that is used in the brain can be applied to the retina because retinal glia differs from glia in the brain. Here, we aimed to find a method for selective gene expression in Müller cells (characteristic glial cells in the retina) and identified Mlc1 as a specific promoter of Müller cells.

Methods

Mlc1-tTA::Yellow-Cameleon-NanotetO/tetO (YC-Nano) mice were used as a reporter line. YC-Nano, a fluorescent protein, was ectopically expressed in the cell type controlled by the Mlc1 promotor. Immunofluorescen

SUBMITTER: Danjo Y 

PROVIDER: S-EPMC8764212 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature