Multi-Omics Profiling Identifies Pathways Associated With CD8⁺ T-Cell Activation in Severe Aplastic Anemia.

ABSTRACT: Severe aplastic anemia (SAA) is an autoimmune disease characterized by immune-mediated destruction of hematopoietic stem and progenitor cells. Autoreactive CD8⁺ T cells have been reported as the effector cells; however, the mechanisms regulating their cell activation in SAA remain largely unknown. Here, we performed proteomics and metabolomics analyses of plasma and bone marrow supernatant, together with transcriptional analysis of CD8⁺ T cells from SAA patients and healthy donors, to find key pathways that are involved in pathogenic CD8⁺ T-cell activation. We identified 21 differential proteins and 50 differential metabolites in SAA patients that were mainly involved in energy metabolism, complement and coagulation cascades, and HIF-1α signaling pathways.

SUBMITTER: You X 

PROVIDER: S-EPMC8764265 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature