Pituitary Somatotroph Adenoma-derived Exosomes: Characterization of Nonhormonal Actions.

ABSTRACT:

Context

The identification and biological actions of pituitary-derived exosomes remain elusive.

Objective

This work aimed to validate production of exosomes derived from human and rat pituitary and elucidate their actions.

Methods

Isolated extracellular vesicles (EVs) were analyzed by Nanoparticle Tracking Analysis (NTA) and expressed exosomal markers detected by Western blot, using nonpituitary fibroblast FR and myoblast H9C2 cells as controls. Exosome inhibitor GW4869 was employed to detect attenuated EV release. Exosomal RNA contents were characterized by RNA sequencing. In vitro and in vivo hepatocyte signaling alterations responding to GH1-derived exosomes (GH1-exo) were delineated by mRNA sequencing. GH1-exo actions on protein synthesis, cAMP (3',5'-cyclic adeno

SUBMITTER: Zhou C 

PROVIDER: S-EPMC8764361 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature